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KMID : 0381120110330050499
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Genes and Genomics
2011 Volume.33 No. 5 p.499 ~ p.499
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Development and characterization of microsatellite markers for genetic analysis of the Korean black scraper, Thamnaconus modestus
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An Hye-Suck
Lee Jang-Wook
Dong Chun-Mae
Noh Jae-Koo
Myeong Jeong-In
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Abstract
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Black scraper is a commercially important oceanodromous fish in Korea. The commercial catch of this fish has decreased continuously since 1990. However, its genetic characteristics have never been studied. For population genetic analysis to assist effective aquaculture and stock management efforts, we isolated and characterized 19 microsatellite loci using an microsatellite-enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. To characterize each locus, 30 individuals from a natural T. modestus population in southern Korea were genotyped. All loci except two, KTm213 and KTm284, were polymorphic, with an average of 15.18 alleles per locus (range, 2?41). The mean observed and expected heterozygosities were 0.67 (range, 0.33?0.97) and 0.79 (range, 0.40?1.00), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at six loci (KTm142, KTm145, KTm158, KTm24, KTm221, and KTm250). This high variability indicates that these microsatellites may be useful for high-resolution studies of population genetics.
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KEYWORD
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Korean black scraper, Thamnaconus modestus, Microsatellite loci, Genetic marker, Heterozygosity
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